Human cytotoxic T-lymphocyte repertoire to influenza A viruses.
Identifieur interne : 001A43 ( Main/Exploration ); précédent : 001A42; suivant : 001A44Human cytotoxic T-lymphocyte repertoire to influenza A viruses.
Auteurs : J. Jameson [États-Unis] ; J. Cruz ; F A EnnisSource :
- Journal of virology [ 0022-538X ] ; 1998.
Descripteurs français
- KwdFr :
- Adulte, Animaux, Antigènes HLA, Antigènes viraux, Cartographie épitopique, Humains, Interféron gamma (biosynthèse), Lignée cellulaire, Lymphocytes T cytotoxiques (immunologie), Mémoire immunologique, Protéines de la matrice virale (immunologie), Protéines virales (immunologie), Réactions croisées, Sialidase (immunologie), Souris, Techniques in vitro, Virus de la grippe A (), Virus de la grippe A (immunologie), Épitopes.
- MESH :
- biosynthèse : Interféron gamma.
- immunologie : Lymphocytes T cytotoxiques, Protéines de la matrice virale, Protéines virales, Sialidase, Virus de la grippe A.
- Adulte, Animaux, Antigènes HLA, Antigènes viraux, Cartographie épitopique, Humains, Lignée cellulaire, Mémoire immunologique, Réactions croisées, Souris, Techniques in vitro, Virus de la grippe A, Épitopes.
English descriptors
- KwdEn :
- Adult, Animals, Antigens, Viral, Cell Line, Cross Reactions, Epitope Mapping, Epitopes, HLA Antigens, Humans, Immunologic Memory, In Vitro Techniques, Influenza A virus (classification), Influenza A virus (immunology), Interferon-gamma (biosynthesis), Mice, Neuraminidase (immunology), T-Lymphocytes, Cytotoxic (immunology), Viral Matrix Proteins (immunology), Viral Proteins (immunology).
- MESH :
- chemical , biosynthesis : Interferon-gamma.
- chemical , immunology : Neuraminidase, Viral Matrix Proteins, Viral Proteins.
- chemical : Antigens, Viral, Epitopes, HLA Antigens.
- classification : Influenza A virus.
- immunology : Influenza A virus, T-Lymphocytes, Cytotoxic.
- Adult, Animals, Cell Line, Cross Reactions, Epitope Mapping, Humans, Immunologic Memory, In Vitro Techniques, Mice.
Abstract
The murine CD8(+) cytotoxic-T-lymphocyte (CTL) repertoire appears to be quite limited in response to influenza A viruses. The CTL responses to influenza A virus in humans were examined to determine if the CTL repertoire is also very limited. Bulk cultures revealed that a number of virus proteins were recognized in CTL assays. CTL lines were isolated from three donors for detailed study and found to be specific for epitopes on numerous influenza A viral proteins. Eight distinct CD8(+) CTL lines were isolated from donor 1. The proteins recognized by these cell lines included the nucleoprotein (NP), matrix protein (M1), nonstructural protein 1 (NS1), polymerases (PB1 and PB2), and hemagglutinin (HA). Two CD4(+) cell lines, one specific for neuraminidase (NA) and the other specific for M1, were also characterized. These CTL results were confirmed by precursor frequency analysis of peptide-specific gamma interferon-producing cells detected by ELISPOT. The epitopes recognized by 6 of these 10 cell lines have not been previously described; 8 of the 10 cell lines were cross-reactive to subtype H1N1, H2N2, and H3N2 viruses, 1 cell line was cross-reactive to subtypes H1N1 and H2N2, and 1 cell line was subtype H1N1 specific. A broad CTL repertoire was detected in the two other donors, and cell lines specific for the NP, NA, HA, M1, NS1, and M2 viral proteins were isolated. These findings indicate that the human memory CTL response to influenza A virus is broadly directed to epitopes on a wide variety of proteins, unlike the limited response observed following infection of mice.
PubMed: 9765409
Affiliations:
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Jameson</name><affiliation wicri:level="1"><nlm:affiliation>Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical Center, Worcester, Massachusetts 01655, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical Center, Worcester, Massachusetts 01655</wicri:regionArea><wicri:noRegion>Massachusetts 01655</wicri:noRegion></affiliation></author><author><name sortKey="Cruz, J" sort="Cruz, J" uniqKey="Cruz J" first="J" last="Cruz">J. Cruz</name></author><author><name sortKey="Ennis, F A" sort="Ennis, F A" uniqKey="Ennis F" first="F A" last="Ennis">F A Ennis</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antigens, Viral</term><term>Cell Line</term><term>Cross Reactions</term><term>Epitope Mapping</term><term>Epitopes</term><term>HLA Antigens</term><term>Humans</term><term>Immunologic Memory</term><term>In Vitro Techniques</term><term>Influenza A virus (classification)</term><term>Influenza A virus (immunology)</term><term>Interferon-gamma (biosynthesis)</term><term>Mice</term><term>Neuraminidase (immunology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Viral Matrix Proteins (immunology)</term><term>Viral Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Antigènes HLA</term><term>Antigènes viraux</term><term>Cartographie épitopique</term><term>Humains</term><term>Interféron gamma (biosynthèse)</term><term>Lignée cellulaire</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Mémoire immunologique</term><term>Protéines de la matrice virale (immunologie)</term><term>Protéines virales (immunologie)</term><term>Réactions croisées</term><term>Sialidase (immunologie)</term><term>Souris</term><term>Techniques in vitro</term><term>Virus de la grippe A ()</term><term>Virus de la grippe A (immunologie)</term><term>Épitopes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-gamma</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Neuraminidase</term><term>Viral Matrix Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antigens, Viral</term><term>Epitopes</term><term>HLA Antigens</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron gamma</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term><term>Protéines de la matrice virale</term><term>Protéines virales</term><term>Sialidase</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Cell Line</term><term>Cross Reactions</term><term>Epitope Mapping</term><term>Humans</term><term>Immunologic Memory</term><term>In Vitro Techniques</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Antigènes HLA</term><term>Antigènes viraux</term><term>Cartographie épitopique</term><term>Humains</term><term>Lignée cellulaire</term><term>Mémoire immunologique</term><term>Réactions croisées</term><term>Souris</term><term>Techniques in vitro</term><term>Virus de la grippe A</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The murine CD8(+) cytotoxic-T-lymphocyte (CTL) repertoire appears to be quite limited in response to influenza A viruses. The CTL responses to influenza A virus in humans were examined to determine if the CTL repertoire is also very limited. Bulk cultures revealed that a number of virus proteins were recognized in CTL assays. CTL lines were isolated from three donors for detailed study and found to be specific for epitopes on numerous influenza A viral proteins. Eight distinct CD8(+) CTL lines were isolated from donor 1. The proteins recognized by these cell lines included the nucleoprotein (NP), matrix protein (M1), nonstructural protein 1 (NS1), polymerases (PB1 and PB2), and hemagglutinin (HA). Two CD4(+) cell lines, one specific for neuraminidase (NA) and the other specific for M1, were also characterized. These CTL results were confirmed by precursor frequency analysis of peptide-specific gamma interferon-producing cells detected by ELISPOT. The epitopes recognized by 6 of these 10 cell lines have not been previously described; 8 of the 10 cell lines were cross-reactive to subtype H1N1, H2N2, and H3N2 viruses, 1 cell line was cross-reactive to subtypes H1N1 and H2N2, and 1 cell line was subtype H1N1 specific. A broad CTL repertoire was detected in the two other donors, and cell lines specific for the NP, NA, HA, M1, NS1, and M2 viral proteins were isolated. These findings indicate that the human memory CTL response to influenza A virus is broadly directed to epitopes on a wide variety of proteins, unlike the limited response observed following infection of mice.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Cruz, J" sort="Cruz, J" uniqKey="Cruz J" first="J" last="Cruz">J. Cruz</name><name sortKey="Ennis, F A" sort="Ennis, F A" uniqKey="Ennis F" first="F A" last="Ennis">F A Ennis</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Jameson, J" sort="Jameson, J" uniqKey="Jameson J" first="J" last="Jameson">J. Jameson</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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